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Beads were washed twice with wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM Imidazole) and the protein finally eluted with elution buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 500 mM Imidazole)

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Difference between melena and hematochezia Difference between melena and hematochezia Melena and hematochezia are both signs of gastrointestinal bleeding, but they differ in stool color, bleeding source, and how long blood remains in the digestive tract