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doi: 10.1007/s00281-015-0531-3 92 PiJShenLYangEShenHHuangDWangRet al

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Enzyme-linked immunosorbent assay Microtiter plates were coated with antigens (mGH and Trx-mGH) diluted in phosphate buffer at 2 g/mL and 4 g/mL, respectively, and incubated overnight at 4 C

These studies highlight a positive influence of the microenvironment on tumour formation

After 24 h, cells were treated with DMSO control or RSL3 (0.11.0 M) and incubated for 7 days to allow for tumor spheroid formation