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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

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It is not a cause and effect relationship, because the factors that lead to the onset of serious diseases such as those we have seen are always many, but it is still an indicator to keep an eye on

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All sorts of closures for the glass vials were considered for antimicrobial protection

doi: 10.2147/IJN.S106289 103 GuntiL.DassR