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The hCMEC/D3 cells (passage 2832) were seeded on collagen coated culture flasks (2.5-3 10 4 /cm 2 ) or glass slides (4 10 4 /cm 2 ) and maintained at 37C with 5% CO 2 exposure in EBM-2 basal medium (Lonza, Walkersville, MD, USA) supplemented with 5% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), chemically defined lipid concentrate (Life Technologies, Carlsbad, CA, USA), growth factors, antibiotic/antimycotic (1:1, Atlanta Biologicals, GA, USA and HEPES (10 mM)

SOURCES: Len Horovitz, MD, pulmonary specialist, Lenox Hill Hospital, New York City

Despite steadily declining rates over the years, hundreds of thousands of Americas Veterans still smoke cigarettes, according to VAs most recent Survey of Veteran Enrollees Health and Use of Health Care Report

While lungs can detoxify themselves, smoking slows down this process

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