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Primary lung fibroblasts (between passages 3 and 7) and HFL-1 cells (between passages 14 and 20) were cultured in tissue culture dishes with DMEM supplemented with 10% fetal calf serum, 100 g/ml penicillin, 250 g/ml streptomycin, and 2.5 g/ml amphotericin B

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[14] Biotin injections must be given by a qualified healthcare professional, such as a doctor or nurse