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Shaking the vial creates mechanical stress that can break the delicate bonds holding the amino acid chains together, a process called denaturation

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Imaging was performed using Plan Apo 60 oil (NA 1.4) objective and Andor DU-897 X-8536 camera of the inverted Nikon Ti microscope (Nikon Corporation, Chiyoda, Tokyo, Japan) setup

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Biochemistry 45 (51): 1518815196

However, many of these models were established using small animals, such as mice, rats and rabbits, which involve different characteristics from the human spine, particularly in terms of disc anatomy and mechanical loading of vertebrae