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B 109 (43), 2020720214 (2005)

In addition to its macronutrients and minerals, soybean has many positive effects on human health due to its contents of oil, protein and isoflavones ( Drought stress has negative effects at the physiological, developmental, and molecular levels in plants , including photosynthesis inhibition, reactive oxygen species (ROS) generation, and cellular tissue and membrane damage (Xu et al., 2010

Guo et al., 2016), they represent a promising disease-modifying therapeutic class

Although the use of primary brain endothelial cells and/or co-culture with other cells would provide a physiologically more relevant environment, bEND.3 cells consistently show a stable barrier function and thus have been widely used in previous studies on BBB permeability to therapeutics 35

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Proteins were separated by 10% SDS-PAGE gel and transferred to the polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# IPVH00010)