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10,752,918 An in vitro method for producing a gene-edited cell comprising transfecting in vitro a cell comprising a Duchenne muscular dystrophy (DMD) gene with a nucleic acid encoding a gene-editing protein, wherein the gene-editing protein comprises: (a) a DNA-binding domain and (b) a nuclease domain and is capable of binding to the DMD gene, wherein: (a) the DNA-binding domain comprises a plurality of repeat sequences and at least one of the repeat sequences comprises the amino acid sequence: LTPvQWAlAwxyzGHGG (SEQ ID NO: 75) and is between 36 and 39 amino acids long, wherein: v is Q, D or E, w is S or N, x is N or H, y is D, A, H, N, K, or G, and z is GGKQALETVQRLLPVLCQD (SEQ ID NO: 670) or GGKQALETVQRLLPVLCQA (SEQ ID NO: 671)

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Turbulent eddies scour the channel bed, and can keep sediment in suspension longer than laminar flow and thus aids in erosion of the stream bottom