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After 30 minutes at 37C, 500 L of cold ethanol was added to the homogenate to precipitate proteins, followed by centrifugation (21,000 g , 4C, 10 minutes), and the supernatant was dried under an N 2 stream at room temperature

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Concordantly, we found that most of the metaphases in WT as well as in ADH5 cells were normal and only few of them presented chromosome damage

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These findings should be further validated in cancer patients undergoing radiation, as the available data are insufficient to support them