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Somewhat surprisingly, all four individual Cys to Ser mutants retained a neutravidin/ palmitoylation signal

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Further pathological staining revealed that the combined treatment reduced tumor proliferation, decreased the number of fibroblasts, and alleviated tumor necrosis (Figure 7G and Supplement Figure 3C-D)

The IP complex was captured by adding 50 l GFP-Trap (ChromoTek, Germany, gta-20) or Anti-FLAG Antibody (Engibody, AT0022), followed by shaking at 4 C for an additional hour

Other prevalent reasons could be due to the lack of vitamins A, B2, B6, vitamin C, D, E, zinc and copper

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