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When the cotyledons fully expanded, VIGS was performed as described previously ( S -nitrosoglutathione reductase1 ( SlGSNOR1 , SGN accession Solyc09g064370.2.1) and RESPIRATORY BURST OXIDASE HOMOLOG 1 ( SlRBOH1 , SGN accession Solyc08g081690.2.1) were amplified using gene-specific primers: SlGSNOR1 , 5-tgctctagaAGCAACCCATTCAGCAAGTC-3 and 5-cgc ggatccTGTTTATGTCCGCAAGTGTC-3 (with Xba I and Bam HI restriction sites)

Proebstle TM, Alm J, Dimitri S, Rasmussen L, Whiteley M, Lawson J,

doi: 10.1016/j.tifs.2017.02.013 19

Liposomal encapsulation of reduced glutathione has been determined to be stable for at least 14 months without refrigeration and remains capable of anti-viral effect

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Sterile packaging ensures each syringe remains contamination-free until use